The following CRISPR/Cas9 fly lines are available through the Bloomington Drosophila Stock Center:
51321 w[1118]; PBac{y[+mDint2] w[GMR.PHb]=U6-tracrRNA}VK00037/CyO, P{Wee-P.ph0}2
51322 y[1] M{w[GMR.PHb]=U6-tracrRNA}ZH-2A w[1118]/FM7c, P{w[+mC]=GAL4-Kr.C}DC1, P{w[+mC]=UAS-GFP.S65T}DC5, sn[+]
51323 y[1] M{vas-Cas9}ZH-2A w[1118]/FM7c
51324 w[1118]; PBac{y[+mDint2]=vas-Cas9}VK00027
51325 w[1118]; PBac{y[+mDint2]=vas-Cas9,U6-tracrRNA}VK00027
51326 y[1] M{vas-Cas9,U6-tracrRNA}ZH-2A w[1118]
The following plasmids are available through Addgene:
- pBS-Hsp70-Cas9 (in pBluescript)
- pHsp70-Cas9 (low copy number version used in Gratz et al., 2013)
- pU6-BbsI-chiRNA (gRNA vector using the U6-2 promoter used in Gratz et al., 2013)
- pHD-DsRed-attP (donor vector used in used in Gratz et al., 2014)
- pHD-DsRed (donor vector used in used in Gratz et al., 2014)
- pHD-ScarlessDsRed (donor vector with a PBac DsRed cassette for scarless marker removal)
- pHD-sfGFP-ScarlessDsRed (donor vector for scarless tagging with sfGFP)
- pHD-3xFLAG-ScarlessDsRed (donor vector for scarless tagging with 3xFLAG)
- pHD-2xHA-ScarlessDsRed (donor vector for scarless tagging with 2xHA)
A number of plasmids are also available with w+ in the backbone for selection against repair events resulting in donor backbone incorporation.
The following plasmids are available through the DGRC:
- pBS-Hsp70-Cas9 (in pBluescript)
- pU6-2-gRNA (gRNA vector using the U6-2 promoter)
- pU6-3-gRNA (gRNA vector using the U6-3 promoter)
- pHD-DsRed-attP (donor vector with loxP flanked DsRed marker used in used in Gratz et al., 2014)
- pHD-DsRed (donor vector with loxP flanked DsRed marker used in used in Gratz et al., 2014)
- pHD-ScarlessDsRed (donor vector with a PBac DsRed cassette for scarless marker removal)
- pHD-sfGFP-ScarlessDsRed (donor vector for scarless tagging with sfGFP)
- pHD-3xFLAG-ScarlessDsRed (donor vector for scarless tagging with 3xFLAG)
- pHD-2xHA-ScarlessDsRed (donor vector for scarless tagging with 2xHA)